Inhibition of SNARE-driven neuroexocytosis by plant extracts |
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Authors: | Chang Hwa Jung Yoo Soo Yang Jun-Seob Kim Yeon-Kyun Shin Jae Sung Hwang Eui Dong Son Hong Hwa Lee Koo Min Chung Jung Mi Oh Jong Hwa Lee Dae-Hyuk Kweon |
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Institution: | 1. School of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, 440-746, South Korea 2. Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, 55455, USA 3. Department of Biochemistry and Biophysics, Iowa State University, Ames, IA, 50011, USA 4. Amorepacific R&D Center, 314-1 Bora-Dong, Yongin, 446-729, South Korea 5. School of Bioresource Sciences, Andong National University, Andong, 760-749, South Korea
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Abstract: | Neuronal soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) proteins mediate membrane fusion between synaptic
vesicle and presynaptic membrane, resulting in neurotransmitter release. SNARE proteins are specific substrates of botulinum
neurotoxins (BoNT) which are now widely used for therapeutic and cosmetic purposes. While BoNT blocks neuroexocytosis by cleaving
SNAREs, inhibiting SNARE assembly process might exert the same effect on neurotransmission. In the present study, some extracts
of 100 plants reduced neurotransmitter release by inhibiting SNARE complex formation in neuronal cells. The extracts effectively
paralyzed muscle of rat phrenic nerve-hemidiaphragm preparation. Our results raise the possibility that SNARE folding inhibitors
from natural resources might replace some special BoNT application fields.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Chang Hwa Jung and Yoo Soo Yang equally contributed to this work. |
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Keywords: | SNARE Neurotransmitter release Plant extracts Botulinum neurotoxin |
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