Mycoplasmal infection of lymphocyte cell cultures: Infection withM. salivarium |
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Authors: | Gerard J McGarrity David M Phillips Akhil B Vaidya |
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Institution: | (1) Institute for Medical Research, Camden, New Jersey;(2) Population Council, Rockefeller University, New York, New York;(3) Department of Microbiology, Hahnemann Medical College, Philadelphia, Pennsylvania |
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Abstract: | Summary Many conclusions concerning cell culture mycoplasmas are based on data from studies in fibroblast cultures. Some conclusions
may not be valid in other types of differentiated cell cultures.M. salivarium was isolated from 35 human lymphocyte cultures (HLC), 34 from the same laboratory. The organism grew to more than 108 colony forming units (CFU) per ml of lymphocyte suspensions and was readily detectable by microbiological culture, uridine
phosphorylase, and uridine/uracil assays. Direct mycoplasmal assays on HLC by DNA fluorochrome staining and scanning electron
microscopy (SEM) yielded artifacts that interfered with diagnosis. For DNA and SEM of HLC, inoculation into indicator cell
cultures is recommended.M. salivarium infection of HLC did not produce any immediate difference in growth rates; however, infected cultures eventually died 14
to 29 passages after infection in contrast to uninfected controls. The same organism in 3T6 fibroblasts effected a 60% decrease
in growth rate. AlthoughM. salivarium is a frequent isolate from the oral cavity, it is a rare cell culture isolate.M. salivarium was able to initiate growth over a wide pH range, grew as well in cell cultures as in cell-free media, and was resistant
to 50 μg per ml of gentamycin, tylocine, kanamycin, and erythromycin. By C0t1/2 analysis,M. salivarium had a genomic molecular weight of 4.2×108 daltons.M. salivarium did not increase chromosome aberrations in one HLC. Some of these results have application to infection of HLC by other mycoplasmal
species.
These studies were supported by contracts NO1-AG-82117 from the National Institute on Aging, NO1-GM-9-2101 from the National
Institute of General Medical Sciences, and Grant RO1-A1-15748 from the National Institute of Allergy and Infectious Diseases. |
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Keywords: | mycoplasma M salivarium lymphocyte cell cultures |
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