The oxidation product of molybdenum cofactor from milk xanthine oxidase |
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| Authors: | R J van Spanning C W Wansell-Bettenhaussen L F Oltmann A H Stouthamer |
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| Affiliation: | Free University, Department of Microbiology, Amsterdam, The Netherlands. |
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| Abstract: | In extracts of acid treated molybdenum cofactor containing xanthine oxidase, fluorescence is maximally developed upon a three hours incubation. Analysis by means of reversed phase HPLC revealed the presence of several fluorescent compounds, the main one being a blue fluorescent compound with an emission maximum of 465 nm when maximal excited at 395 nm at a neutral pH. Definite proof is presented that this compound is the oxidation product of the molybdenum cofactor. The remaining fluorescent products are shown to be pterin-derivatives, yielding predominantly pterin-6-carboxylic acid upon permanganate oxidation. Purified oxidation product of molybdenum cofactor however, didn's yield a fluorescent derivative at all upon treatment with permanganate. |
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