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| 口虾蛄肠道细菌种群多样性的分子分析 | |
| 引用本文： | 王海青,曲艳梅,刘斌.口虾蛄肠道细菌种群多样性的分子分析[J].中国微生态学杂志,2010,22(4):317-320,324. |
| 作者姓名： | 王海青 曲艳梅 刘斌 |
| 作者单位： | 1. 中国科学院海洋研究所,实验海洋生物学重点实验室,山东,青岛,266071;中国科学院研究生院,北京,100039 2. 中国科学院海洋研究所,实验海洋生物学重点实验室,山东,青岛,266071 3. 中国科学院海洋研究所,实验海洋生物学重点实验室,山东,青岛,266071;中国科学院北京基因组研究所,北京,101300 |
| 基金项目： | 致谢：谨向本论文的资助机构科技部863计划（2007AA09Z444）,中科院百人计划,中科院海洋功能基因组团队计划表示感谢. |
| 摘 要： | 目的探讨口虾蛄肠道细菌种群的多样性。方法通过不依赖于分离培养的分子生物学分析方法,以直接提取虾蛄肠道细菌的总DNA为模板经过PCR扩增16S DNA,然后经与T载体连接建立质粒文库。用限制性内切酶(BsuRⅠ和Hin6Ⅰ)对阳性克隆的PCR扩增产物进行限制性酶切片段长度多态性(RFLP)分析,选取有代表性的克隆进行测序。结果16S DNA序列通过CLUSTALX进行多序列比对及NCBI数据库中的BLAST分析后发现口虾蛄肠道细菌主要分成4类:未培养细菌,未培养支原体科细菌,未培养δ变形菌和马特斯支原体。结论口虾蛄肠道细菌种类较为简单,且多为未培养的细菌。 |
| 关 键 词： | 口虾蛄 肠道细菌 16S DNA 克隆文库 限制性片段长度多态性 |
Molecular analysis of gut bacterial communities of Oratosquilla oratoria |
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| WANG Hai-qing,QU Yan-mei,LIU Bin.Molecular analysis of gut bacterial communities of Oratosquilla oratoria[J].Chinese Journal of Microecology,2010,22(4):317-320,324. | |
| Authors: | WANG Hai-qing QU Yan-mei LIU Bin |
| Institution: | 1.Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;2.Graduate University of Chinese Academy of Sciences,Beijing 100039,China;3.Beijing Institute of Genomics,Chinese Academy of Sciences,Beijing 101300,China) |
| Abstract: | Objective To investigate the intestinal bacterial diversity in Oratosquilla oratoria.Method Culture-independent method was used,and 16S DNA was amplified via PCR from total genomic DNA extracted from intestinal microorganisms,with clone libraries constructed later. Positive clones were digested by BsuRⅠand Hin6Ⅰrespectively,and then representative clones were sequenced.Result All sequences fell into four groups through phylogenetic analysis of 16S DNA of these microbial communities using CLUSTAL X and BLAST. The result revealed that most of these bacteria were uncultured bacteria,uncultured Mycoplasmataceae bacterium,uncultured δ-proteobacterium and Mycoplasma moatsii. Conclusion The gut of Oratosquilla oratoria have very low bacterial diversity,and most of the bacteria are uncultured bacterium clones. |
| Keywords: | 16S DNA |
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