| 荧光物质浸泡标记稀有鮈鲫和彭泽鲫仔、稚鱼 |
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| 引用本文： | 欧阳斌,常剑波.荧光物质浸泡标记稀有鮈鲫和彭泽鲫仔、稚鱼[J].水生生物学报,1999(4). |
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| 作者姓名： | 欧阳斌 常剑波 |
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| 作者单位： | 中国科学院水生生物研究所!武汉４３００７２ |
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| 基金项目： | 国务院三峡办和中国长江三峡工程开发总公司资助!1－03－02－01 |
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| 摘 要： | 研究了茜素络合物和盐酸四环素对稀有鮈鲫和彭泽鲫仔、稚鱼进行浸泡标记的方法和效果。茜素络合物在浸泡浓度５０—１００ｍｇ／Ｌ，浸泡时间持续６—２４ｈ的范围内，对两种鱼都有很好的标记效果；盐酸四环素在浸泡浓度１５０—２５０ｍｇ／Ｌ，浸泡时间１２—２４ｈ的范围内，对彭泽鲫有较好的标记效果，但对稀有 鲫有极高的致死率。在荧光显微镜下观察，茜素络合物呈橘红色、盐酸四环素标记区呈金黄色荧光反应。
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| 关 键 词： | 茜素络合物 盐酸四环素 标记 仔、稚鱼 |
USING FLUORESCENT SUBSTANCE TO MARK LARVAE AND JUVENILE OF GOBIOCYPRIS RARUS AND CARASSIUS AURATUS PENGZE VAR |
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| Ouyang Bin and Chang Jianbo.USING FLUORESCENT SUBSTANCE TO MARK LARVAE AND JUVENILE OF GOBIOCYPRIS RARUS AND CARASSIUS AURATUS PENGZE VAR[J].Acta Hydrobiologica Sinica,1999(4). |
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| Authors: | Ouyang Bin and Chang Jianbo |
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| Abstract: | The present paper deals with methods and effects of using alizarincomplexone (ALC)and chlorydrate of tetracycline (CHTC) to mark larval and juvenileGobiocypris rarus and Carassius auratus pengze Var. To immerse the two species in50-100mg/ L ALC solutions for 6-24h, a well-defined mark on otolith was induced.A well-defined mark on otolith was also observed when immersing larval Carassiusauratus pengze Var in 150-250mg/L CHTC solutions for 12-24h. However, onlyimmersing -caused mortality was observed in G. rarus when immersed in CHTCsolution. suggesting that TC may not be suitable for being used as marking solution.Under UV light,the mark of ALC in the otoliths is a scarlet-pink fluorescent zone,and that of CHTC is a yellow fluorescent zone. |
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| Keywords: | Alizarin complexone Chlorhydrate of tetracycline Mark Larvae andjuvenile fish |
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