A protocol for rapid micropropagation of endangered Isoplexis |
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Authors: | Pedro Pérez-Bermúdez H. Ulrich Seitz Isabel Gavidia |
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Affiliation: | (1) Present address: Department of Plant Biology, Faculty of Pharmacy, University of Valencia, Av. V.A. Estellés s/n, 46100 Burjassot, (Valencia), Spain;(2) Center of Plant Molecular Biology, Auf der Morgenstelle 1, 72076 Tübingen, Germany |
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Abstract: | Summary An efficient protocol for large-scale micropropagation of Isoplexis canariensis (L.) Loud., I. chalcantha Svent, and O'Shan., and I. isabelliana (Webb and Berth.) Masf. (Scrophulariaceae) is reported. Multiple shoots were obtained from shoot tips and nodal segments isolated from seedlings when cultured under varying conditions. Factors such as nutrient media, concentration of growth regulators, and type of induction medium (liquid or solid) strongly influenced shoot proliferation and development. Multiple well-developed shoots were obtained after induction on solid media containing Murashige and Skoog full-strength salts and low cytokinin concentrations. By changing the major salt formulation to half strength and employing liquid media the morphogenic parameters evaluated were affected negatively. Many shoots rooted spontaneously in hormone-free media and plants grew successfully in the greenhouse. Flowering was observed 6 mo. after ex vitro cultures were established. |
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Keywords: | endangered species Isoplexis hyperhydricity micropropagation multiple shoots nodal segments shoot tips |
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