应用叶绿素荧光法测定微藻生物量的方法

为了探求叶绿素荧光值与海洋微藻生物量的关系,利用TD-700型叶绿素荧光仪测定了6种典型海洋赤潮藻在不同生长期(指数生长期和稳定生长期)时的叶绿素荧光值,同时用传统方法测定细胞密度,且利用Leica Qwin软件测量细胞大小。结果显示:微藻在同一生长时期细胞密度与叶绿素荧光值存在极显著的正相关关系,不同生长时期细胞密度也与单位叶绿素荧光值存在较显著正相关关系。因此,利用叶绿素荧光仪测定微藻生物量的方法是切实可行的,具有快捷方便、灵敏度高、可靠性强的优点。

A method of determining microalgal biomass

In order to understand the relationship between chlorophyll fluorescence and biomass of microalgae, the fluorescences of six typical marine red tide microalgae in different growth phases (exponential growth phase and steady growth phase) are measured using TD-700 fluorometer; meanwhile the cell density is counted under light microscrope and cell size is determined using Leica Qwin software. The results show that there is a significantly positive relationship between fluorescent value and the cell density in the same growth phase and also in the different growth phases. The results suggest that chlorophyll fluorescence is a quick and reliable method to determine microalgal biomass.