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Extranuclear mutant ofSchizophyllum commune
Authors:J Ne?vera
Institution:(1) Department of Genetics, Faculty of Sciences, Charles University, 128 44 Prague 2;(2) Present address: Department of Molecular Biology and Genetics, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4
Abstract:A mutant of the hymenomyceteSchizophyllum commune was isolated which, owing to an extranuclear mutation, did not utilize acetate as the sole carbon source for growth. The growth of the mutant on glucose minimal medium was completely inhibited by sodium azide but was resistant to the effect of 2,4-dinitrophenol or oligomycin. Its endogenous respiration was cyanide-sensitive and was stimulated by 2,4-dinitrophenol to a considerably smaller degree than that of the wild-type strain. The experimental results obtained with this mutant suggest a defect in aerobic phosphorylation.
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