Analysis of involvement of the 3'-untranslated regions in regulating mRNA stability for vitellogenin,cyanoprotein alpha,and cyanoprotein beta from the bean bug,Riptortus clavatus |
| |
Authors: | Wojtasek Hubert Miura Ken Shinoda Tetsuro Chinzei Yasuo |
| |
Affiliation: | Department of Medical Zoology, Mie University, School of Medicine, Tsu, Japan. wojtasek@uni.opole.pl |
| |
Abstract: | The degradation of the 3'-untranslated regions (UTRs) of vitellogenin, cyanoprotein alpha, and cyanoprotein beta from the bean bug, Riptortus clavatus, was analyzed in vitro. The degradation pattern was similar for all three RNAs, with a high degradation rate in non-diapausing adult insects and no degradation in the fifth instar nymphs and in diapausing adults, and was not correlated with the expression levels of these three proteins. Proteins binding to the 3'-UTRs were detected in polysomal and cytosolic extracts. These factors, however, were present in all developmental stages. The abundance of the polysomal factor showed little variation, but the cytosolic factor was enriched in adult insects. Cross-competition experiments demonstrated that the same factors bound to all three RNAs with similar affinity. The pattern of degradation, presence of the binding factors in all stages, and their inability to distinguish between the target sequences indicate that the 3'-UTRs do not participate in controlling the expression of these three proteins. |
| |
Keywords: | cyanoprotein hexamerin juvenile hormone diapause RNA binding protein |
本文献已被 PubMed 等数据库收录! |
|