Diversity in nucleotide acquisition by antigenically similar Chlamydia psittaci of avian origin |
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Authors: | Grant McClarty Huizhou Fan Arthur A. Andersen |
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Affiliation: | Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada; Avian Diseases Research Unit, National Animal Disease Center, Agricultural Research Services, US Department of Agriculture, Ames, Iowa, USA |
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Abstract: | Abstract Two different nucleic acid precursor utilization patterns were obtained for five avian isolates of Chlamydia psittaci . Three of the isolates bahaved in a manner similar to that previously described, showing total dependency on the host cell for ribonucleoside triphosphates and being unable to utilize medium-supplied thymidine. In contrast, the other two isolates were incapable of taking pyrimidine ribonucleotides from the host cell and they could efficiently utilize medium-supplied thymidine. These unusual isolates were resistant to 5-fluorouridine while the other three isolates were sensitive. Of the five isolates only 6BC was sensitive to sulfonamides. The five isolates were divided into two groups by comparing the Alu I restriction endonuclease patterns obtained following digestion of the major outer membrane protein (OMP1) gene, amplified by the polymerase chain reaction. The OMP1 genotyping results were confirmed by serotyping. |
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Keywords: | Chlamydia Nucleotide metabolism Thymidine OMP1 |
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