Construction of ploidy series of Saccharomyces cerevisiae by the plasmid YCplac33-GHK |
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| Authors: | Lihua Hou Xiaoyang Li Cong Wang Xiaohong Cao Haiyong Wang |
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| Institution: | 1. Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Ministry of Education, Tianjin, 300457, People’s Republic of China
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| Abstract: | An effective approach, using the plasmid YCplac33-GHK, is developed to construct a ploidy series of Saccharomyces cerevisiae. YCplac33-GHK harbors the HO gene under the control of galactose-inducible promoter and KanMX4 as the selective marker. The simple method can solve the problem of industrial applications of strains with resistance genes. |
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