TPA-induced multinucleation of a mesenchymal stem cell-like clone is mediated primarily by karyokinesis without cytokinesis, although cell-cell fusion also occurs |
| |
Authors: | Yoshida Keiichiro Obata Shuichi Ono Michio Esaki Michiyo Maejima Tatsuo Sawada Hajime |
| |
Affiliation: | Department of Histology and Cell Biology, Yokohama City University School of Medicine, Fukuura 3-9, Kanazawa-ku, Yokohama, Kanagawa-ken 236-0004, Japan. k6552@yokohama-cu.ac.jp |
| |
Abstract: | The 5F9A cell, which is a mesenchymal stem cell-like clone established from rat bone marrow substrate adherent cells, can differentiate into adipocytes and osteoblasts in vitro under the appropriate conditions. Multinucleated cells could be also induced by 12-O-tetradecanoylphorbol 13-acetate (TPA) in 5F9A cells. This effect was mediated by protein kinase C. Possible mechanisms of multinucleation by TPA were hypothesized to be either karyokinesis without cytokinesis or cell-cell fusion. By observation using time-lapse phase-contrast microscopy, we determined that the multinucleated cells were generated mainly by karyokinesis without cytokinesis. Cell fusion was studied using time-lapse photography, and confocal laser scanning microscopy using two differentially labeled cells. These techniques demonstrated that multinucleated 5F9A cells could be produced by cell fusion, albeit at a low frequency. We conclude that multinucleated 5F9A cells are formed primarily by karyokinesis without cytokinesis, although some cells are also formed by cell-cell fusion. |
| |
Keywords: | Plasmodium Syncytium Mesenchymal stem cell Cell– cell fusion Phorbol ester TPA Multinucleation Protein kinase C Cytokinesis Nuclear division |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|