| 秦川牛和中国荷斯坦牛POU1F1基因多态性研究 |
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| 引用本文： | 严林俊,刘波,房兴堂,陈宏,张润锋,鲍斌,张海军.秦川牛和中国荷斯坦牛POU1F1基因多态性研究[J].遗传,2006,28(11):1371-1375. |
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| 作者姓名： | 严林俊 刘波 房兴堂 陈宏 张润锋 鲍斌 张海军 |
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| 作者单位： | 1. 徐州师范大学细胞与分子生物学研究所,徐州 221116; 2. 西北农林科技大学动物科技学院 ;陕西省农业分子生物学重点实验室, 杨凌712100 ; |
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| 基金项目： | 江苏省高校自然科学基金，陕西省自然科学基金，国家高技术研究发展计划(863计划)，西北农林科技大学校科研和教改项目 |
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| 摘 要： | 采用PCR-RFLP技术研究了秦川牛(QQ)和中国荷斯坦牛(HC)共计218头个体POU1F1基因的多态性。结果表明: 秦川牛及中国荷斯坦牛群体POU1F1-HinfⅠ基因座的451 bp 的PCR产物经限制性酶HinfⅠ消化后表现多态, 其等位基因A/B频率分别为0.232/0.768、0.132/0.868; 两个群体AA、AB和BB 3种基因型的频率分别为0.030/0.403/0.567、0.007/0.251/0.742。在该基因座秦川牛群体处于Hardy-Weinberg平衡状态, 中国荷斯坦牛群体处于不平衡状态。它们在该基因座的杂合度、有效等位基因数、Shannon信息熵、多态信息含量分别为0.356/1.553/0.541/0.292、0.229/1.297/0.390/0.203; 秦川牛群体的位点杂合度、有效等位基因数、Shannon信息熵、多态信息含量均大于中国荷斯坦牛群体。
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| 关 键 词： | POU1F1 秦川牛 多态性 PCR-RFLP 荷斯坦牛 |
| 文章编号： | 0253-9772(2006)11-1371-05 |
| 收稿时间： | 2005-12-30 |
| 修稿时间： | 2006-03-02 |
Analysis of POU1F1 Gene Polymorphisms in Qinchuan Cattle and Chinese Holstein Cattle |
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| YAN Lin-Jun,LIU Bo,FANG Xin-Tang,ZHANG Run-Feng,BAO Bin,ZHANG Hai-Jun,CHEN Hong.Analysis of POU1F1 Gene Polymorphisms in Qinchuan Cattle and Chinese Holstein Cattle[J].Hereditas,2006,28(11):1371-1375. |
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| Authors: | YAN Lin-Jun LIU Bo FANG Xin-Tang ZHANG Run-Feng BAO Bin ZHANG Hai-Jun CHEN Hong |
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| Institution: | 1. Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou 221116, China; 2. College of Animal Science ;and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China ; |
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| Abstract: | PCR-RFLP was applied to analyze the polymorphisms of P0U1F1 gene in 218 Qinchuan cattle (QQ) and Chinese Holstein cattle (HC). Results demonstrated Hint I polymorphisms in the 451 bp PCR product in the two populations. The frequencies of alleles A/B in QQ and HC populations were 0.232/0.768 and 0.132/0.868, respectively. The frequencies of three genotypes AA, AB and BB were 0.030/0.403/0.567 and 0.007/0.251/0.742, respectively. Qinchuan cattle population was at Hardy-Weinberg equilibrium at this locus, but Chinese Holstein cattle population was not. The gene heterozygosity/effective allele gene number/Shannon information entropy /polymorphism information content of Qinchuan cattle and Chinese Holstein cattle populations were listed for 0.356/1.553/0.541/0.292 and 0.229/1.297/0.390/0.203, respectively. All indices were higher in the Qinchuan cattle population. |
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| Keywords: | POU1F1 PCR-RFLP |
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