Calspectin (fodrin or nonerythroid spectrin)-actin interaction: a possible involvement of 4.1-related protein |
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Authors: | K Kanda T Tanaka K Sobue |
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Affiliation: | 2. Nephrology Section, Department of Internal Medicine, Ghent University Hospital, Ghent, Belgium |
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Abstract: | The calspectin/actin complex extracted from the bovine brain membrane crosslinks F-actin, resulting in the increasing viscosity of F-actin determined by low-shear viscometry. We demonstrated the presence of a protein factor in this complex, which regulated the calspectin-F-actin interaction in a Ca2+- and calmodulin-dependent manner. Erythrocyte protein 4.1, but not synapsin I, mimics the function of this brain factor using a reconstitution system including purified calspectin, calmodulin and F-actin. In the brain complex, the Mr 120,000 and the Mr 80,000/77,000 polypeptides were detected to crossreact with anti-protein 4.1 antibody. |
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