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Initiation factor promoted reassociation of eukaryotic ribosomal subunits
Authors:R E Wettenhall  D P Leader  I G Wool
Affiliation:1. Laboratory AGEIS EA 7407, Team Tools for e-Gnosis Medical & Labcom CNRS/UGA/OrangeLabs Telecom4Health, Faculty of Medicine, Université Grenoble Alpes, F-38700, La Tronche, France;2. The National Natural History Collections, The Hebrew University of Jerusalem, 91404, Jerusalem, Israel;1. Institute of Medical Physics and Biophysics, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany;2. Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine, 10115 Berlin, Germany;3. Institute for Integrative Neuroanatomy, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany;4. Institute of Cell Biology and Neurobiology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Charitéplatz 1, 10117 Berlin, Germany;5. Institute of Neuroscience, Lobachevsky University of Nizhny Novgorod, pr. Gagarina 24, Nizhny Novgorod, Russian Federation;6. Max Delbrück Center for Molecular Medicine, 13092 Berlin, Germany;7. Center for Molecular Biology of Heidelberg University (ZMBH) and German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, 69120 Heidelberg, Germany;8. Humboldt Universität zu Berlin, Faculty of Life Sciences, 10117 Berlin, Germany;9. Max Planck Institute for Molecular Genetics, Microscopy and Cryo-Electron Microscopy Service Group, 14195 Berlin, Germany;10. Department of Neuroscience and Cell Biology, Rutgers University-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA;11. Core Unit Bioinformatics, Berlin Institute of Health, 10117 Berlin, Germany;1. Biotech Research and Innovation Centre, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark;2. Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW) and Department of Neuroscience, Faculty of Health and Medical Science, University of Copenhagen, 2200 Copenhagen, Denmark;3. Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark;4. Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany;5. Wallenberg Center for Molecular Medicine, Department of Experimental Medical Science, Lund University, 22184 Lund, Sweden
Abstract:A factor present in rat liver cytosol caused 40S and 60S subunits to reassociate, forming 80S ribosomes. Reassociation required this factor, an RNA template (poly U), and aminoacyl-tRNA (Phe-tRNA); it was enhanced by GTP. Factor-promoted reassociation was not inhibited by puromycin.
Keywords:
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