| 雪松聚球藻金属硫蛋白的纯化及其性质的研究 |
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| 引用本文： | 夏建荣 戴玲芬. 雪松聚球藻金属硫蛋白的纯化及其性质的研究[J]. 水生生物学报, 1998, 22(2): 112-118 |
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| 作者姓名： | 夏建荣 戴玲芬 |
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| 作者单位： | 汕头大学科学研究院能源与环境科学研究所!515063，中国科学院水生生物研究所!武汉，430072，中国科学院水生生物研究所!武汉，430072 |
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| 基金项目： | 中国科学院水生生物研究所所长基金!950502 |
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| 摘 要： | 在雪松聚球藻的培养基中逐渐增加氯化镉的浓度以诱导藻细胞内金属硫蛋白的合成，经Ｓｅｐｈａｄｅｘ Ｇ－５０，ＤＥＡＥ－ｃｅｌｌｕｌｏｓｅ和Ｓｅｐｈａｄｅｘ Ｇ－２５柱层析，获得的ＭＴ没有亚型，经ＳＤＳ－ＰＡＧＥ分析是高度均一的。每个蛋白分子约含５个Ｃｄ原子，１０个巯基，分子量约为７．６ＫＤ，等电位ｐＨ４．５左右，半胱氨酸含量约占总氨基酸量的１５．５％，还含有少量的芳香族氨基酸，ＭＴ的最大紫外吸收在２５
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| 关 键 词： | 雪松聚球藻 金属硫蛋白 纯化 性质 |
PURIFICATION AND CHARACTERIZATION OF METALLOTHIONEIN FROM SYNECHOCOCCUS CEDRORUM |
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| Xia Jianrong. PURIFICATION AND CHARACTERIZATION OF METALLOTHIONEIN FROM SYNECHOCOCCUS CEDRORUM[J]. Acta Hydrobiologica Sinica, 1998, 22(2): 112-118 |
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| Authors: | Xia Jianrong |
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| Abstract: | The metallothionein (MT) is induced from synechococcus cedrorum growing in medium added gradually with cadmium chloride,and purified and characterized by means of Sephadex G-50. DEAE-cellulose (DE-52) and Sephadex G-25 colunm chromatography. The results show MT has no isoforms and is demonstrated to be homogenous by SDS-PAGE. Each molecule of MT contains 10 mercapto groups and combines with 5 cadmium atoms. Its molechlar weight is about 7.6KD,with isoelectric point being about pH4.5.Its cysteine resides account for 15.5% of all amino acid resides. The MT with a characteristic absorption maxium at 250nm is poorly stable with a large decrease of the absorption peak in low pH, and contains a little aromatic acid. |
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| Keywords: | Synechococcus cedrorum Metallothionein (MT) |
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