银鲫复合种外源遗传物质整入的RAPD分析

在优化ＲＡＤＰＤ检测条件的基础上，采用４０对随机引物，比较分析了银复合物，异育银鲫和兴国红鲤相互间扩增ＤＮＡ片段的异同性。总的来说，银鲫复合种和异育银鲫具有基本一致的扩增产物，而与兴国红鲤的扩增产物多数不同，相似率分析表明，银鲫复合种与兴国红鲤之间的相似率为３１．６％，异育银鲫与兴国红鲤之间的相似率为２８．６％。在分析中，除发现银鲫复合种，异育银鲫与兴国红鲤间共有扩增片段外，还发现了银鲫复合种与兴

RAPD ANALYSIS OF INCORPORATION OF HETEROLOGOUS GENETIC MATERIALS IN MULTIPLE SPECIES OF SILVER CRUCIAN CARP

The similarities and differences of amplified DNA fragments among themultiple species of silver crucian carp, allogynogenetic silver crucian carp and redcommon carp (Cyprinus carpio) were analysed through the RAPD technique optimizedin this system. Forty random primers of 10 nucleotide long sequence were used in theRAPD analysis. Generally, there existed similar amplified products between themultiple species and allogynogenetice silver crucian carp, whereas most of the productswere obviously different in red common carp. The similarity of the amplified DNAbands between the multiple species and red common carp was 31.6%, and thesimilarity between allogynogenetic silver crucian carp and red common carp was28.6%. Some amplified DNA bands were discovered to be shared by the multiple species, allgynogenetic silver carp and red common carp, and some bands wererevealed to be shared between the multiple species and red common carp. Moreover,a few of specific bands were found only in the multiple species. The results not onlyprovided new molecular evidences for incorporation of heterologous genetic materialsin the multiple species, but also demonstrated that heterologous genetic materials inthe multiple species were more than that in allogynogenetic silver crucian carp.