High-performance liquid chromatography-mass spectrometry method for the determination of paroxetine in human plasma |
| |
Authors: | Zhu Zhimeng Neirinck Len |
| |
Institution: | Pharmascience, Suite 100, 6111 Royalmount Ave, Montreal, Quebec, Canada H4P 2T4. zzhupharmascience.com |
| |
Abstract: | A rapid and specific liquid chromatographic mass spectrometric (LC-MS-MS) method has been developed for the determination of paroxetine in human plasma. The procedure involves a liquid-liquid extraction of paroxetine and fluoxetine (internal standard) with cyclohexane-ethyl acetate. The standard curve was linear over a working range of 0.2-50 ng/ml. The lower limit of quantitation was 0.2 ng/ml. No endogenous compounds were found to interfere with the analysis. The absolute recovery was 70.8% for paroxetine and 84.1% for the internal standard. The accuracy of inter-assay and intra-assay accuracy was in the ranges -4.8 to -0.5% and -3.4 to 4.8%, respectively. This method proved to be suitable for bioequivalence studies by being simple, selective and reproducible. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|