The genomic architecture of disease resistance in lettuce |
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Authors: | Leah K McHale Maria José Truco Alexander Kozik Tadeusz Wroblewski Oswaldo E Ochoa Kirsten A Lahre Steven J Knapp Richard W Michelmore |
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Institution: | (1) The Genome Center and Department of Plant Sciences, University of California, Davis, CA 95616, USA;(2) Institute of Plant Breeding, Genetics and Genomics, Center for Applied Genetic Technologies, University of Georgia, Athens, GA 30602, USA |
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Abstract: | Genbank and The Compositae Genome Project database, containing over 42,000 lettuce unigenes from Lactuca sativa cv. Salinas and L. serriola accession UC96US23 were mined to identify 702 candidate genes involved in pathogen recognition (RGCs), resistance signal transduction, defense responses, and disease susceptibility. In addition, to identify sequences representing
additional sub-families of nucleotide binding site (NBS)-leucine-rich repeat encoding genes; the major classes of resistance
genes (R-genes), NBS-encoding sequences were amplified by PCR using degenerate oligonucleotides designed to NBS sub-families specific
to the subclass Asteridae, which includes the Compositae family. These products were cloned and sequenced resulting in 18
novel NBS sequences from cv. Salinas and 15 novel NBS sequences from UC96US23. Using a variety of marker technologies, 294
of the 735 candidate disease resistance genes were mapped in our primary mapping population, which consisted of 119 F7 recombinant inbred lines derived from an interspecific cross between cv. Salinas and UC96US23. Using markers shared across
multiple genetic maps, 36 resistance phenotypic loci, including two new loci for resistance to downy mildew and two quantitative
trait loci for resistance to anthracnose were positioned onto the reference map to provide a global view of the genomic architecture
of disease resistance in lettuce and to identify candidate genes for resistance phenotypes. The majority but not all of the
resistance phenotypes were genetically associated with RGCs.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
An erratum to this article can be found at |
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