Crystallographic characterization of a Cu,Zn superoxide dismutase from Photobacterium leiognathi |
| |
Authors: | S M Redford D E McRee E D Getzoff H M Steinman J A Tainer |
| |
Affiliation: | Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037. |
| |
Abstract: | Crystals of a copper-zinc superoxide dismutase from Photobacterium leiognathi, a luminescent marine bacterium that is the species-specific symbiont of the ponyfish, have been obtained from 2-methyl-2,4-pentanediol solutions. The space group was determined using screenless small-angle precession photographs, and was confirmed by analyzing area detector diffraction data with the XENGEN programs for indexing and refinement. The crystals are monoclinic, space group C2 (a = 126.4 A, b = 87.0 A, c = 44.4 A, beta = 92.8 A), and have two 32,000 Mr dimers per asymmetric unit. The crystals diffract to at least 2.7 A resolution, are resistant to radiation damage, and are suitable for determination of the structure by X-ray diffraction. |
| |
Keywords: | |
|
|