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The use of potassium iodide equilibrium density gradient centrifugation in the purification of RNA for hybridization with nonreiterated DNA sequences.
Authors:F J Gonzalez  C T Garrett  D Wiener  M D Caine
Institution:Department of Biochemistry, Texas Tech, University School of Medicine, Lubbock, Texas 79409 USA
Abstract:A new instrument has been developed that is able to monitor enzyme activities in chromatography column eluents automatically. The instrument consists of a simple stopped-flow instrument, equipped with a multishutoff valve to direct reagents and eluent flow. Assays of the eluate may be done at present intervals. The instrument may also be used for monitoring enzyme activities in perfasates as well as for monitoring the elution of metabolites.
Keywords:To whom reprint requests should be addressed: Present address is Department of Pathology  The George Washington University  2300 Eye Street  N  W    Washington  D  C  20037  
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