Degradation of naphthalene by thermophilic bacteria <Emphasis Type="Italic">via</Emphasis> a pathway,through protocatechuic acid |
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Authors: | Audrius Bubinas Gražina Giedraitytė Lilija Kalėdienė Ona Nivinskiene Rita Butkiene |
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Institution: | (1) Department of Plant Physiology and Microbiology, Faculty of Natural Sciences, Vilnius University, LT-03101 Vilnius, Lithuania;(2) Institute of Chemistry, LT-01108 Vilnius, Lithuania |
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Abstract: | A number of thermophilic bacteria capable of utilizing naphthalene as a sole source of carbon were isolated from a high-temperature
oilfield in Lithuania. These isolates were able to utilize several other aromatic compounds, such as anthracene, benzene,
phenol, benzene-1, 3-diol, protocatechuic acid as well. Thermophilic isolate G27 ascribed to Geobacillus genus was found to have a high aromatic compound degrading capacity. Spectrophotometric determination of enzyme activities
in cell-free extracts revealed that the last aromatic ring fission enzyme in naphthalene biotransformation by Geobacillus sp. G27 was inducible via protocatechuate 3, 4-dioxygenase; no protocatechuate 4, 5-dioxygenase, protocatechuate 2, 3-dioxygenase activities were detected.
Intermediates such as o-phthalic and protocatechuic acids detected in culture supernatant confirmed that the metabolism of
naphthalene by Geobacillus sp. G27 can proceed through protocatechuic acid via ortho-cleavage pathway and thus differs from the pathways known for mesophilic bacteria. |
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Keywords: | Thermophilic bacteria Geobacillus Naphthalene Dioxygenases |
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