| 大鼠发情期和间情期下丘脑ghrelin mRNA的表达 |
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| 引用本文： | 张文龙,童德文,邢福姗. 大鼠发情期和间情期下丘脑ghrelin mRNA的表达[J]. 动物学杂志, 2009, 44(4): 28-33 |
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| 作者姓名： | 张文龙 童德文 邢福姗 |
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| 作者单位： | 西北农林科技大学动物医学院,陕西,杨凌,712100;西北农林科技大学动物医学院,陕西,杨凌,712100;西北农林科技大学动物医学院,陕西,杨凌,712100 |
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| 基金项目： | 西北农林科技大学人才基金项目(No.01140502) |
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| 摘 要： | 为探索下丘脑ghrelin mRNA及GnRH mRNA在大鼠(Rattus norregicus)发情期和间情期的表达特点,通过外部观察和阴道涂片相结合的方法确定发情期和间情期,将12只未经产SD雌性大鼠分为2组,即发情期组和间情期组,每组6只。取动物下丘脑,用实时荧光RT-PCR方法检测ghrelin mRNA和GnRH mRNA的表达丰度。结果表明,间情期组大鼠下丘脑ghrelin mRNA的表达丰度显著高于发情期组(P<0.01);间情期组大鼠下丘脑GnRH mRNA的表达丰度显著低于发情期组(P<0.01)。研究发现,下丘脑ghrelin mRNA和GnRH mRNA在发情期与间情期具不同的表达模式,提示ghrelin可能在下丘脑水平上对GnRH mRNA的表达具下调作用。
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| 关 键 词： | Ghrelin mRNA 发情期 间情期 实时荧光RT-PCR 大鼠 |
| 收稿时间： | 2008-12-18 |
| 修稿时间： | 2009-04-29 |
Expression of Ghrelin mRNA in the Hypothalamus of Rats during Oestrous Cycle |
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| ZHANG Wen-Long,TONG De-Wen and XING Fu-Shan. Expression of Ghrelin mRNA in the Hypothalamus of Rats during Oestrous Cycle[J]. Chinese Journal of Zoology, 2009, 44(4): 28-33 |
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| Authors: | ZHANG Wen-Long TONG De-Wen XING Fu-Shan |
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| Affiliation: | College of Veterinary Medicine;Northwest A&F University;Yangling;Shaanxi 712100;China |
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| Abstract: | This study was designed to investigate the expression of ghrelin mRNA in the hypothalamus of rats at the estrus and diestrus.Twelve virginal rats were classified into 2 groups as the estrus(n=6) and diestrus(n=6) which were determined by vaginal smears daily.All animals were killed by decapitation,and samples of hypothalamic tissue were obtained to detect the value of ghrelin and GnRH mRNA by real-time RT-PCR.Results showed that expression of ghrelin mRNA in the hypothalamus changed with the oestrous cycle,... |
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| Keywords: | Ghrelin mRNA Estrus Diestrus Real-time RT-PCR Rats |
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