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| 怀山药醇提取物抗DPPH自由基活性研究 | |
| 引用本文： | 赵东保,刘绣华,汪汉卿. 怀山药醇提取物抗DPPH自由基活性研究[J]. 天然产物研究与开发, 2005, 17(3): 272-274 |
| 作者姓名： | 赵东保 刘绣华 汪汉卿 |
| 作者单位： | 1. 中国科学院兰州化学物理研究所OSSO国家重点实验室,兰州,730000;河南大学化学化工学院,开封,475001 2. 河南大学化学化工学院,开封,475001 3. 中国科学院兰州化学物理研究所OSSO国家重点实验室,兰州,730000 |
| 基金项目： | CommitteeofScienceandTechnologyofHenanProvince(No.0123031000)，MinistryofScienceandTechnologyofthePeoplesRepublicofChina(999290106)，HenanInnovationProjectForUniversityProminentResearchTalents(2004KYCX018) |
| 摘 要： | 将怀山药乙醇提取物采用溶剂萃取的方法，分成极性不同的五个部分，并首次用DPPH(2，2-diphenyl-1-picrylhydrazyl)方法测定各部分的抗自由基活性，发现乙酸乙酯萃取部分活性最强，氯仿萃取部分次之，再其次是正丁醇和水溶性部分。乙酸乙酯和氯仿萃取部分较强的抗自由基活性主要归因于其中所含的多酚类成分。同时利用薄层层析(TLC)、紫外光谱(UV)、^13C核磁共振(NMR)技术及显色反应对酚性成分进行了定性检验，并用Folin-Denis法测定了各萃取部位中酚性成分含量，发现抗自由基活性与萃取物中多酚性成分含量有一定的相关性。因而在评价怀山药质量时，其中所含的酚性成分不应忽视。 |
| 关 键 词： | DPPH自由基 怀山药 活性研究 ^13C核磁共振 自由基活性 乙酸乙酯 酚性成分 成分含量 乙醇提取物 溶剂萃取 薄层层析 紫外光谱 定性检验 显色反应 水溶性 正丁醇 多酚类 相关性 萃取物 测定 氯仿 |
Scavenging Activity of Dioscorea opposita thumb to DPPH Free Radicals |
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| ZHAO Dong-Bao,LIU Xiu-hua,WANG Han-qing. Scavenging Activity of Dioscorea opposita thumb to DPPH Free Radicals[J]. Natural Product Research and Development, 2005, 17(3): 272-274 | |
| Authors: | ZHAO Dong-Bao LIU Xiu-hua WANG Han-qing |
| Affiliation: | ZHAO Dong-bao 1,2,LIU Xiu-hua 2*,WANG Han-qing 1* |
| Abstract: | The ethanol extracts of the crude drug Dioscorea opposita thumb(Huai Shanyao) were divided into five fractions by liquid-liquid partitions by the increasing polarity. The study on DPPH free radical scavenging activities showed that the ethyl acetate fraction has the strongest scavenging activity to DPPH free radicals.The next is chloroform fraction,followed by n-butanol and aqueous fraction. The stronger scavenging activity to DPPH free radicals of the ethyl acetate and chloroform fractions should be mainly due to the polyphenolics contained. The presence of phenolic compounds in the extracts was also analyzed qualitatively by TLC, 13C NMR,color reaction and quantitatively by spectrophotometric method(Folin-Denis). |
| Keywords: | Dioscorea opposita thumb radical scavenging activities polyphenolics |
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