A Drosophila ribosomal protein functions in mammalian cells. |
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| Authors: | C G Maki D D Rhoads J J Diaz and D J Roufa |
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| Institution: | Division of Biology, Kansas State University, Manhattan 66506. |
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| Abstract: | A cDNA expression vector encoding Drosophila ribosomal protein S14 was transfected into cultured Chinese hamster ovary (CHO) cells that harbor a recessive RPS14 emetine resistance mutation. Transformants synthesized the insect mRNA and polypeptide and consequently displayed an emetine-sensitive phenotype. These observations indicate that the insect protein was accurately expressed and correctly assembled into functional mammalian 40S ribosomal subunits. |
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