Evidence that Tn5565, which includes the enterotoxin gene in Clostridium perfringens, can have a circular form which may be a transposition intermediate |
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| Authors: | Sigrid Brynestada Per Einar Granuma |
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| Institution: | Department of Pharmacology, Microbiology and Food Hygiene, Norwegian College of Veterinary Medicine, PO Box 8146, Dep., N-0033 Oslo, Norway |
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| Abstract: | The Clostridium perfringens enterotoxin gene is on a transposon-like element, Tn5565, integrated in the chromosome in human food poisoning strains. The flanking IS elements, IS1470 A and B, are related to IS30. The IS element found in the transposon, IS1469, is related to IS200 and has been found upstream of cpe in all Type A strains. PCR and sequencing studies from cell extracts and plasmid isolations of C. perfringens indicate that Tn5565 can form a circular form with the tandem repeat (IS1470)2, similar to the transposition intermediates described for a number of IS elements. |
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| Keywords: | Clostridium perfringens Enterotoxin Transposition IS1470 IS1469 |
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