Callus, Organogenesis and Plantlet Formation in Tissue Cultures of Clitoria ternatea |
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Authors: | LAKSHMANAN, K. K. DHANALAKSHMI, S. |
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Affiliation: | Department of Botany, Bharathiar University Coimbatore 641 046, India |
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Abstract: | Decoated seeds of Clitoria ternatea L. germinated on Murashigeand Skoog (Physiologia Plantarum 1962, 15, 47397) basalmedium (BM) and differentiated callus and bipolar embryoids(two-step method) in low frequency. Calluses developed on lateralroots [BM+KN(0.1 mg 11)], on roots and hypocotyls [BM+KN(0.5mg 11)], and on roots [BM+KN+IAA (0.5 mg 11 ofeach)]. On basal medium with KN (0.5 mg 11) and withKN+IAA (0.5 mg11 of each), multiple shoot buds and embryoids(one-step method) were differentiated directly on split hypocotylsand roots. In the former, shoot buds developed even on unsplithypocotyls. Rhizogenesis on isolated shoot buds occurred efficientlyin BM+indole butyric acid (IBA 0.1 mg 11) and BM+IAA(0.1 mg 11 and 0.5 mg 11). Profuse direct embryoidsand shoot buds developing on root systems are interesting morphogeneticphenomena rarely reported. Clitoria ternatea L., callus, embryoids, multiple shoot buds, regeneration |
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