Monocyte tumoricidal activity and tumor necrosis factor production in patients with malignant brain tumors |
| |
Authors: | Barbara P Barna Lisa R Rogers Mary Jane Thomassen Gene H Barnett Melinda L Estes |
| |
Institution: | (1) Department of Immunopathology, The Cleveland Clinic Foundation, One Clinic Center, 9500 Euclid Avenue, 44195-5131 Cleveland, OH, USA;(2) Department of Neurology, The Cleveland Clinic Foundation, One Clinic Center, 9500 Euclid Avenue, 44195-5131 Cleveland, OH, USA;(3) Department of The Research Institute, The Cleveland Clinic Foundation, One Clinic Center, 9500 Euclid Avenue, 44195-5131 Cleveland, OH, USA;(4) Department of Neurosurgery, The Cleveland Clinic Foundation, One Clinic Center, 9500 Euclid Avenue, 44195-5131 Cleveland, OH, USA;(5) Department of Pathology, The Cleveland Clinic Foundation, One Clinic Center, 9500 Euclid Avenue, 44195-5131 Cleveland, OH, USA |
| |
Abstract: | Summary Monocyte-mediated tumoricidal activity, tumor necrosis factor (TNF ) secretion and gene expression were examined in astrocytoma patients, patients with other types of brain tumors (primary or metastatic), and normal individuals. The spontaneous monocyte-mediated tumoricidal activity of either patient group against an astrocytoma cell line was significantly greater than normal. There was no difference between patient groups. When monocytes were stimulated with lipopolysaccharide in vitro, tumoricidal activity increased in all patient groups. Patient monocyte activity tested shortly (48 h) after surgery was not different from that before surgery. Both spontaneous and stimulated monocyte cytocidal activities were tumor-cell-restricted: melanoma and astrocytoma cells were equally susceptible but non-neoplastic glial cells were not affected. Examination of monocyte TNF secretion and mRNA expression indicated that patient activity was comparable to or greater than normal. These results demonstrate that, despite steroid therapy, circulating monocytes in astrocytoma and other brain tumor patients retain intact functional activity.Supported in part by grant CA-49 950 from the National Cancer Institute (B.P.B) and grant 1454-HL from the National Institutes of Health (M.L.E.) |
| |
Keywords: | Monocyte Tumor necrosis factor Brain tumors |
本文献已被 SpringerLink 等数据库收录! |
|