| 杂交酶及其相关技术研究进展 |
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| 引用本文： | 刘连碧,朱春宝,朱宝泉.杂交酶及其相关技术研究进展[J].生命科学,2000,12(4):185-188. |
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| 作者姓名： | 刘连碧 朱春宝 朱宝泉 |
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| 作者单位： | 上海医药工业研究院生化制药事业部,上海,200040 |
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| 摘 要： | 根据杂交酶研究目的以及构建杂交酶方法,文中总结近年来杂交酶研究的成果,将杂交酶的应用分为以下几个主要的方面改变酶的非催化特性;创造新活性的酶;研究蛋白质的结构和功能的关系等.还介绍了DNA序列改组、表达克隆、分子筛选、人造细胞样区室筛选基因等技术的进展以及这些技术在构建杂交酶方面的应用.
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| 关 键 词： | 杂交酶 DNA序列改组 表达克隆 分子筛选 人造细胞样区室基因筛选 |
| 文章编号： | 1004-0374(2000)04-0185-04 |
| 修稿时间： | 1999年6月22日 |
Research advances in hybrid enzymes and relative techniques |
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| LIU Lian-Bi,ZHU Chun-Bao,ZHU Bao-Quan.Research advances in hybrid enzymes and relative techniques[J].Chinese Bulletin of Life Sciences,2000,12(4):185-188. |
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| Authors: | LIU Lian-Bi ZHU Chun-Bao ZHU Bao-Quan |
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| Abstract: | The research advances in hybrid enzymes have been summarized in this review according to the reseaxch purpose and construction methods. The hybrid enzyme techniques have been used to modify enzyme's non-catalysis characters, create novel enzymes, study the relationship between function and structure of protein. Meanwhile, DNA shufIling, expression cloning, molecular screening, and man made cell-like compartment techniques are aJso introduced. |
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| Keywords: | hybrid enzyme DNA shuffling expression cloning molecular screening man-made cell-like compartment |
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