Development and application of a simple loop-mediated isothermal amplification method on rapid detection of Listeria monocytogenes strains |
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| Authors: | Li Wang Yue Li Jin Chu Zhenbo Xu Qingping Zhong |
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| Affiliation: | (1) Food Safety Key Laboratory of Guangdong Province, College of Food Science, South China Agricultural University, Guangzhou, 510642, China;(2) School of Food Science and Nutrition, Faculty of Mathematics and Physical Sciences, University of Leeds, Leeds, LS2 9JT, United Kingdom;(3) College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, 510640, China;(4) Department of Microbial Pathogenesis, Dental School, University of Maryland-Baltimore, Baltimore, MD 21201, USA; |
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| Abstract: | A loop-mediated isothermal amplification (LAMP) method for rapid detection of the food-borne L. monocytogenes strains had been developed and evaluated in this study. The optimal reaction condition was 65°C for 45 min, with the detection limit as 1 pg DNA/tube and 100 CFU/reaction. Application of the established LAMP assay was performed on 182 food-borne L. monocytogenes strains using a rapid procedure and easy result confirmation, with the sensitivity of LAMP versus PCR assays as 96.7% (176/182) and 91.2% (166/182), respectively; with 100% specificity, positive predictive value (PPV) and negative predictive value (NPV) for both assays. |
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