Fine-mapping of <Emphasis Type="Italic">qRfg2</Emphasis>, a QTL for resistance to <Emphasis Type="Italic">Gibberella</Emphasis> stalk rot in maize |
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Authors: | Dongfeng?Zhang Yongjie?Liu Yanling?Guo Qin?Yang Jianrong?Ye Shaojiang?Chen Email author" target="_blank">Mingliang?XuEmail author |
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Institution: | (1) National Maize Improvement Center of China, China Agricultural University, 2 West Yuanmingyuan Road, Beijing, 100193, People’s Republic of China; |
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Abstract: | Stalk rot is one of the most devastating diseases in maize worldwide. In our previous study, two QTLs, a major qRfg1 and a minor qRfg2, were identified in the resistant inbred line ‘1145’ to confer resistance to Gibberella stalk rot. In the present study, we report on fine-mapping of the minor qRfg2 that is located on chromosome 1 and account for ~8.9% of the total phenotypic variation. A total of 22 markers were developed
in the qRfg2 region to resolve recombinants. The progeny-test mapping strategy was developed to accurately determine the phenotypes of
all recombinants for fine-mapping of the qRfg2 locus. This fine-mapping process was performed from BC4F1 to BC8F1 generations to narrow down the qRfg2 locus into ~300 kb, flanked by the markers SSRZ319 and CAPSZ459. A predicted gene in the mapped region, coding for an auxin-regulated
protein, is believed to be a candidate for qRfg2. The qRfg2 locus could steadily increase the resistance percentage by ~12% across different backcross generations, suggesting its usefulness
in enhancing maize resistance against Gibberella stalk rot. |
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