Enzymatic degradation of granular potato starch by <Emphasis Type="Italic">Microbacterium aurum</Emphasis> strain B8.A |
| |
Authors: | Fean?D?Sarian Rachel?M?van der?Kaaij Slavko?Kralj Dirk-Jan?Wijbenga Doede?J?Binnema Email author" target="_blank">Marc?J?E?C?van der?MaarelEmail author Lubbert?Dijkhuizen |
| |
Institution: | (1) Present address: Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands;(2) TNO Quality of Life, Utrechtseweg 48, 3704 HE Zeist, The Netherlands;(3) Present address: Institute Technology Bandung, Jl Ganesha 10, Bandung, 40132, Indonesia;(4) Present address: Genencor International B.V., Archimedesweg 30, 2333 CN Leiden, The Netherlands; |
| |
Abstract: | Microbacterium aurum strain B8.A was isolated from the sludge of a potato starch-processing factory on the basis of its ability to use granular
starch as carbon- and energy source. Extracellular enzymes hydrolyzing granular starch were detected in the growth medium
of M. aurum B8.A, while the type strain M. aurum DSMZ 8600 produced very little amylase activity, and hence was unable to degrade granular starch. The strain B8.A extracellular
enzyme fraction degraded wheat, tapioca and potato starch at 37 °C, well below the gelatinization temperature of these starches.
Starch granules of potato were hydrolyzed more slowly than of wheat and tapioca, probably due to structural differences and/or
surface area effects. Partial hydrolysis of starch granules by extracellular enzymes of strain B8.A resulted in large holes
of irregular sizes in case of wheat and tapioca and many smaller pores of relatively homogeneous size in case of potato. The
strain B8.A extracellular amylolytic system produced mainly maltotriose and maltose from both granular and soluble starch
substrates; also, larger maltooligosaccharides were formed after growth of strain B8.A in rich medium. Zymogram analysis confirmed
that a different set of amylolytic enzymes was present depending on the growth conditions of M. aurum B8.A. Some of these enzymes could be partly purified by binding to starch granules. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|