Comparative mapping of the oat <Emphasis Type="Italic">Dw6/dw6</Emphasis> dwarfing locus using NILs and association with vacuolar proton ATPase subunit H |
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Authors: | Email author" target="_blank">Stephen?J?MolnarEmail author Julie?T?Chapados Sivakala?Satheeskumar Charlene?P?Wight Bonnie?Bancroft Winson?Orr Doris?E?Luckert Solomon?Kibite |
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Institution: | (1) Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Central Experimental Farm, 960 Carling Ave., Ottawa, ON, K1A 0C6, Canada;(2) Lacombe Research Centre, Agriculture and Agri-Food Canada, 6000 C and E Trail, Lacombe, AB, T4L 1W1, Canada |
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Abstract: | Seven pairs of oat near-isogenic lines (NILs) (Kibite in Crop Sci 41:277–278, 2001) contrasting for the Dw6 dwarfing gene were used to test for correlation between tall/dwarf phenotype and polymorphic genotype using restriction fragment
length polymorphism (RFLP) and other molecular markers selected from the Kanota × Ogle (K×O) (Wight et al. in Genome 46:28–47,
2003) and Terra × Marion (De Koeyer et al. in Theor Appl Genet 108:1285–1298, 2004) recombination maps. This strategy located the Dw6/dw6 locus to a small chromosomal region on K×O linkage group (LG) KO33, near or at a putative RFLP locus aco245z. Aco245z and
other tightly linked flanking markers have potential for use in marker-assisted selection (MAS), and PCR-based markers were
developed from several of these. RFLP genotyping of the Dw6 NILs indicated that 13 of the 14 individual lines were homogeneously maternal or paternal for a large genomic region near
Dw6/dw6, an unexpected result for NILs. The cDNA clone aco245 codes for a vacuolar proton ATPase subunit H, a potential candidate
gene for Dw6. Vacuolar proton ATPase enzymes have a central role in plant growth and development and a mutation in subunit C is responsible
for the det3 dwarfing mutation in Arabidopsis thaliana (Schumacher et al. in Genes Dev 13:3259–3270, 1999). Aco245 affords the potential of designing highly precise diagnostic markers for MAS for Dw6. The Dw6 NILs have potential utility to investigate the role of vacuolar proton ATPases in growth and development in plants. |
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