首页 | 本学科首页   官方微博 | 高级检索  
     


Coordinate-based colocalization analysis of single-molecule localization microscopy data
Authors:Sebastian?Malkusch,Ulrike?Endesfelder,Justine?Mondry,Márton?Gelléri,Peter?J.?Verveer,Mike?Heilemann  author-information"  >  author-information__contact u-icon-before"  >  mailto:m.heilemann@uni-wuerzburg.de"   title="  m.heilemann@uni-wuerzburg.de"   itemprop="  email"   data-track="  click"   data-track-action="  Email author"   data-track-label="  "  >Email author
Affiliation:1.Biotechnology and Biophysics,Julius-Maximilians-University Würzburg,Würzburg,Germany;2.Department of Systemic Cell Biology,Max Planck Institute of Molecular Physiology,Dortmund,Germany
Abstract:Colocalization of differently labeled biomolecules is a valuable tool in fluorescence microscopy and can provide information on biomolecular interactions. With the advent of super-resolution microscopy, colocalization analysis is getting closer to molecular resolution, bridging the gap to other technologies such as fluorescence resonance energy transfer. Among these novel microscopic techniques, single-molecule localization-based super-resolution methods offer the advantage of providing single-molecule coordinates that, rather than intensity information, can be used for colocalization analysis. This requires adapting the existing mathematical algorithms for localization microscopy data. Here, we introduce an algorithm for coordinate-based colocalization analysis which is suited for single-molecule super-resolution data. In addition, we present an experimental configuration for simultaneous dual-color imaging together with a robust approach to correct for optical aberrations with an accuracy of a few nanometers. We demonstrate the potential of our approach for cellular structures and for two proteins binding actin filaments.
Keywords:
本文献已被 PubMed SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号