Ion mobility spectrometry for microbial volatile organic compounds: a new identification tool for human pathogenic bacteria |
| |
Authors: | Melanie Jünger Wolfgang Vautz Martin Kuhns Lena Hofmann Siobhán Ulbricht Jörg Ingo Baumbach Michael Quintel Thorsten Perl |
| |
Institution: | 1.Department of Anesthesiology, Emergency and Intensive Care Medicine,University of G?ttingen,G?ttingen,Germany;2.Leibniz-Institute for Analytical Sciences—ISAS—e.V.,Dortmund,Germany;3.Institute for Medical Microbiology, Center for Hygiene and Human Genetics,University of G?ttingen,G?ttingen,Germany;4.KIST Europe—Korea Institute of Science and Technology Europe,Saabrücken,Germany |
| |
Abstract: | Presently, 2 to 4 days elapse between sampling at infection suspicion and result of microbial diagnostics. This delay for
the identification of pathogens causes quite often a late and/or inappropriate initiation of therapy for patients suffering
from infections. Bad outcome and high hospitalization costs are the consequences of these currently existing limited pathogen
identification possibilities. For this reason, we aimed to apply the innovative method multi-capillary column–ion mobility
spectrometry (MCC-IMS) for a fast identification of human pathogenic bacteria by determination of their characteristic volatile
metabolomes. We determined volatile organic compound (VOC) patterns in headspace of 15 human pathogenic bacteria, which were
grown for 24 h on Columbia blood agar plates. Besides MCC-IMS determination, we also used thermal desorption–gas chromatography–mass
spectrometry measurements to confirm and evaluate obtained MCC-IMS data and if possible to assign volatile compounds to unknown
MCC-IMS signals. Up to 21 specific signals have been determined by MCC-IMS for Proteus mirabilis possessing the most VOCs of all investigated strains. Of particular importance is the result that all investigated strains
showed different VOC patterns by MCC-IMS using positive and negative ion mode for every single strain. Thus, the discrimination
of investigated bacteria is possible by detection of their volatile organic compounds in the chosen experimental setup with
the fast and cost-effective method MCC-IMS. In a hospital routine, this method could enable the identification of pathogens
already after 24 h with the consequence that a specific therapy could be initiated significantly earlier. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|