Effect of hydrocortisone and dopamine on activity of beta-glucuronidase in lung parenchyma during experimental septic shock]

An effect of hydrocortisone and dopamine on beta-glucuronidase activity in the lung tissue in experimental septic shock was analysed. The study involved 30 mongrel dogs. Septic shock was induced with i.v. E. coli endotoxin. Total and free activity of beta-glucuronidase was determined with Talalay et al. technique in pulmonary parenchyma homogenate, mitochondrial-lysosomal fraction and supernatant. It was found, that hydrocortisone and dopamine given together increase the stability of the pulmonary lysosomes indicating a value of such management of the septic shock in man.