Switching the mode of sucrose utilization by <Emphasis Type="Italic">Saccharomyces cerevisiae</Emphasis> |
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Authors: | Fernanda Badotti Marcelo G Dário Jr" target="_blank">Sergio L AlvesJr Maria Luiza A Cordioli Luiz C Miletti Pedro S de Araujo Boris U Stambuk |
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Institution: | 1.Departamento de Bioquímica,Centro de Ciências Biológicas, Universidade Federal de Santa Catarina,Florianópolis,Brazil;2.Departamento de Bioquímica,Instituto de Química, Universidade de S?o Paulo,S?o Paulo,Brazil;3.Departamento de Produ??o Animal e Alimentos,Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina,Lages,Brazil |
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Abstract: | Background Overflow metabolism is an undesirable characteristic of aerobic cultures of Saccharomyces cerevisiae during biomass-directed processes. It results from elevated sugar consumption rates that cause a high substrate conversion
to ethanol and other bi-products, severely affecting cell physiology, bioprocess performance, and biomass yields. Fed-batch
culture, where sucrose consumption rates are controlled by the external addition of sugar aiming at its low concentrations
in the fermentor, is the classical bioprocessing alternative to prevent sugar fermentation by yeasts. However, fed-batch fermentations
present drawbacks that could be overcome by simpler batch cultures at relatively high (e.g. 20 g/L) initial sugar concentrations.
In this study, a S. cerevisiae strain lacking invertase activity was engineered to transport sucrose into the cells through a low-affinity and low-capacity
sucrose-H+ symport activity, and the growth kinetics and biomass yields on sucrose analyzed using simple batch cultures. |
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