Distribution of glycoconjugates on the plasma membrane and on membranes of the open-canalicular system in human platelets |
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Authors: | H Kawakami H Hirano |
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Institution: | (1) Department of Anatomy, Kyorin University School of Medicine, 181 Mitaka, Tokyo, Japan |
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Abstract: | Summary Distribution of carbohydrate moieties in the membrane system of the human blood platelet was studied by electron microscopy employing lectins as a probe. Glutaraldehyde-fixed platelets were treated with biotinylatedlectins (ConA, RCA, WGA, PNA, SBA, DBA and UEA-1) and labeled with horseradish peroxidase-conjugated avidin. Among the lectins used, ConA bound uniformly to the plasma membrane as well as to the membrane of the opencanalicular system (OCS). Other lectins showed more or less reduced binding on the OCS membrane compared with that on the plasma membrane, indicating that there exist regional differences in the distribution pattern of glycoconjugates in the membrane system of the platelet. The relationship of the distribution pattern of the glycoconjugates with the distribution of the major platelet glycoproteins GPIb and GPIIbIIIa is discussed. |
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