Rapid transport of phospholipids across the plasma membrane of Leishmania infantum |
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Authors: | Araújo-Santos José Ma Gamarro Francisco Castanys Santiago Herrmann Andreas Pomorski Thomas |
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Affiliation: | Instituto de Parasitologi;a y Biomedicina López-Neyra, C.S.I.C., Granada, Spain. |
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Abstract: | The internalization of fluorescent phospholipid analogs of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), and sphingomyelin (SM) in Leishmania infantum promastigotes was studied. We observed a rapid inward redistribution of NBD-PC, -PE, and -PS across the plasma membrane at 28 and 4 degrees C. This internalization was shown to be independent of the endocytic activity of parasites. Rapid inward movement was coupled to an energy-dependent transporter because it was almost inhibited by depletion of cellular ATP and was blocked after pretreatment with N-ethylmaleimide (NEM). In contrast, NBD-SM traverses the plasma membrane by passive flip-flop. By comparing this pattern of phospholipid transbilayer movement with those known from other eukaryotic cells, candidate lipid transporters are discussed. |
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Keywords: | Parasites Leishmania Lipid translocation NBD Phospholipid |
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