In vitro propagation of Amaryllis belladonna |
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Authors: | M. H. De Bruyn D. I. Ferreira M. M. Slabbert J. Pretorius |
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Affiliation: | (1) Vegetable and Ornamental Plant Research Institute, Private Bag X293, 0001 Pretoria, Republic of South Africa;(2) Research Centre for Plant Biotechnology, Private Bag X293, 0001 Pretoria, USA;(3) Department of Botany, Rand Afrikaans University, P.O. Box 524, 2000 Johannesburg, RSA |
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Abstract: | Amaryllis belladonna L. plants were multiplied successfully by means of tissue culture techniques. Different plant parts were tested as explant material, but plantlets could only be generated from the twin-scales and immature scapes. These in vitro-formed plantlets were divided into four parts and used for further multiplication. The twin-scale explants had the highest multiplication rate when a medium with 22.2 M benzyladenine and 0.54 M naphthaleneacetic acid was used. The sucrose concentration played an important role in the initiation of new plantlets, and the best results were obtained when a sucrose concentration of 2–3% was used. Anatomical observations were made during the initiation of the new plantlets.Abbreviations BA benzyladenine - NAA naphthaleneacetic acid - Benomyl (methyl [1-[(butylamino) carbonyl]-1H-benzimidazol-2-yl] carbamate) - Folpet (2-[(trichloromethyl)thio]-H-isoindole-1,3(2H)-dione phthalimide(I)) |
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Keywords: | Belladonna lily Cape belladonna March lily micropropagation sucrose tissue culture twin-scales |
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