用表面等离子体共振技术筛选与禽流感病毒基质蛋白M1相互作用的蛋白

目的：筛选与禽流感病毒基质蛋白M1相互作用的蛋白。方法：表达禽流感病毒基质蛋白M1，经Ni^2＋柱亲和层析纯化，用表面等离子体共振（SPR）技术捕获BHK-21细胞裂解液中与M1相互作用的细胞蛋白，并进行质谱分析。结果：获得了纯度在85%以上的基质蛋白M1，并利用此蛋白捕获到宿主细胞肌球蛋白重链6。结论：肌球蛋白重链6与禽流感病毒基质蛋白M1可能存在体外相互作用。

Screening of Proteins Interacting with M1 Protein of Avian Influenza Virus by SPR

Objective： To find out the proteins of host cell interacting with the purified avian influenza virus matrix protein M1,which could give a clue to avian influenza pathogenesis. Methods： The soluble M1 with His-tag was expressed in E.coli BL21（DE3）, and then purified by Ni^2＋ affinity chromatography. The surface plasmon resonance biomolecular interaction analysis was used in search of the proteins in host cell BHK-21 which may bind M1 protein. The proteins interacting with M1 were collected for MALDI-TOF MS. Results： We obtained high purity recombinant M1 protein, which is easy to screen its interactants in total cell lysate by BIAcore technique. Mascot research results showed that myosin-6 coold interact with M1 protein. Conclusion： Myosin-6 was found to interact with M1 protein in vitro. Further studies are expected to focus on the role of myosin-6 in avian influenza viral infection and illness progression.