Extraction of indole alkaloids fromCatharanthus roseus by using supercritical carbon dioxide |
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Authors: | Huen Lee Won-Hi Hong Ji-Ho Yoon Kyu-Min Song Sang-Soo Kwak Jang-Ryol Liu |
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Affiliation: | (1) BioProcess Engineering Center, KAIST, 373-1, Kusung-dong, Yusung-gu, 305-701 Taejon, Korea;(2) Plant Cell Biology Lab., Genetic Engineering Research Institute, KIST, Taedok Science Town, P.O. Box 17, Taejon, Korea |
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Abstract: | Summary Indole alkaloids, particularly vindoline and catharanthine, were extracted from the leaves ofCatharanthus roseus by supercritical extraction with CO2. The contents of vindoline and catharanthine in the extracts were determined by HPLC and identified by LC/MS. About 52 %(w/w) of the initial vindoline content, 1.5 mg vindoline/g dry wt leaves, was recovered after extracting this material for 10 h with the CO2 flow rate of 400 ml/min at 40°C and 150 bar. Vindoline concentration in the extract was 67 %(w/w). |
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