微生物转化洛伐他汀条件的初步研究

以本实验室筛出的一株菌ST2710为出发菌，研究不同培养条件对转化率的影响。结果发现，菌株ST2710在种子培养基进行预培养，再转入转化培养基中培养2d后，加入洛伐他汀溶液1.0mL(10mg／mL)后再培养5d，产物的产率可达43．41％；就四种溶剂溶解底物条件进行了研究，结果发现用二甲基甲酰胺溶解洛伐他汀，产物产率为最高，并且转化作用不需要底物的诱导；以单独的菌丝体和上清液转化洛伐他汀时均低于培养液对洛伐他汀的转化作用。

Preliminary studies on conditions for microbial conversion of lovastatin

The optimal medium and the fermentation conditions of converting lovastatin by strain ST2710 were investigated. The results showed that strain ST2710 was preinoculated in seed media for two days, then 1mL of the seed culture was incubated into biotransformation media. After two days of growth, 1.0mL of calibration lovastatin was added and the incubation was continued for another five days. The productivity was 43.41%. The effects of different organic solvents on productivity were also investigated. And it was found the system of bioconversion required no induction with lovastatin; the rate of bioconversion with suspended cells was better than that with washed mycelium or the broth.