Separation and detection of closely related peptides by micellar electrokinetic chromatography coupled with electrospray ionization mass spectrometry using the partial filling technique |
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| Institution: | 1. Faculty of Science, Himeji Institute of Technology, Kamigori, Hyogo 678-12, Japan;2. Kaneka Techno Research Co. Ltd., 1-2-80, Yoshida-cho, Hyogo-ku, Kobe 652, Japan;1. Department of Pharmaceutical Chemistry, School of Pharmacy, University of Kansas, 2095 Constant Avenue, Lawrence, Kansas 66047;2. Department of Chemistry, Kansas State University, 213 CBC Building, Manhattan, Kansas 66506;3. Department of Medicinal Chemistry, School of Pharmacy, University of Kansas, 2095 Constant Avenue, Lawrence, Kansas 66047;1. Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovakia;2. Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovakia;1. University of Washington, Seattle, WA;2. Providence Everett Regional Medical Center, Everett, WA;1. DWI – Leibniz Institute for Interactive Materials e.V;2. Institute for Technical and Macromolecular Chemistry, RWTH Aachen University, Forckenbeckstr. 50, D-52056 Aachen, Germany;3. Department of Plastic, Hand- and Reconstructive Surgery, Hannover Medical School, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany;4. Aquatic Ecology and Centre for Water and Environmental Research (ZWU), University of Duisburg-Essen, Universitätstr. 5, D-45141 Essen, Germany;5. Zschimmer & Schwarz Mohsdorf GmbH & Co KG, Chemnitztalstrasse 1, D-09217 Burgstädt, Germany;6. Technical Chemistry I and Center for Nanointegration Duisburg-Essen (CENIDE), University of Duisburg-Essen, Universitätsstr. 5-7, D-45141 Essen, Germany |
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| Abstract: | Closely related peptides such as neurotensin and angiotensin analogues were separated by capillary zone electrophoresis using a nonionic surfactant, sucrose monododecanoate, as a micelle forming reagent. These peptides were detected by an on-line coupled mass spectrometer using an electrospray ionization interface. However, the presence of the micelles in the separation solution drastically reduced the sensitivity of the mass spectrometer. Therefore, a partial filling technique was employed to prevent the micelles from entering the mass spectrometric interface. A part of the capillary from the injection end was filled with the micellar solution in this technique. Analytes passed through the micellar zone during the electrophoresis and when the separated analytes reached the detection end of the capillary, the micellar zone was still behind the analyte zones, because the nonionic surfactant moved very slowly in acidic conditions. Thus the technique was very useful for mass spectrometric detection for CE when the micellar solution was employed for separation. The optimization of separation and detection conditions was investigated. |
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