Reduction of wall adsorption in capillary zone electrophoresis of a basic single-chain antibody fragment by a cationic polymeric buffer additive |
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| Affiliation: | 1. Institute of Analytical Chemistry, University of Vienna, Währingerstrasse 38, A-1090 Vienna, Austria;2. Institute of Biochemistry, Medical Faculty, University of Vienna, Dr. Bohrgasse 3, A-1030 Vienna, Austria;1. School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China;2. CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China;3. Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China;1. Department of Statistics, Pusan National University Hospital, Gudeok-ro 179, Seo-gu, Busan 49241, Republic of Korea;2. Department of Laboratory Medicine, Hallym University College of Medicine, Kangnam Sacred Heart Hospital, Singil-ro 1, Yeongdeungpo-gu, Seoul 07441, Republic of Korea;3. Department of Rheumatology, Kosin University College of Medicine, Gamcheon-ro 262, Seo-gu, Busan 49267, Republic of Korea;4. Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Yonsei-ro 50, Seodaemun-gu, Seoul 03722, Republic of Korea;5. Department of Internal Medicine, Kosin University College of Medicine, Gamcheon-ro 262, Seo-gu, Busan 49267, Republic of Korea;6. Department of Internal Medicine, Yonsei University College of Medicine, Yonsei-ro 50, Seodaemun-gu, Seoul 03722, Republic of Korea;1. Engineering Research Center for Nanophotonics & Advanced Instrument, Ministry of Education, School of Physics and Electronic Science, East China Normal University, North Zhongshan Rd. 3663, Shanghai, 200062, PR China;2. Joint Institute of Advanced Science and Technology, East China Normal University, Shanghai, 200062, PR China;1. Guangdong Provincial Engineering Technology Research Center for Optical Agricultural, College of Materials and Energy, South China Agricultural University, China;2. Department of Physics, University of Texas at Arlington, Arlington, TX, 76019, USA;1. Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, China;2. Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan |
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| Abstract: | Reduction of adsorptive protein-wall interactions by poly(diallyldimethyl ammonium chloride), a permanently cationic polymer, at a concentration of 0.5% (w/v) is demonstrated for a basic single-chain antibody fragment (scFv, pI about 9.5) even in the range of physiological pH of around 7. The polymer additive forms a positively charged layer at the silica surface which reverses electroosmosis and leads to electrostatic repulsion of the positively charged basic protein. |
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