High-performance liquid chromatographic analysis of Peptide T in rabbit plasma with on-line column enrichment |
| |
| Institution: | 1. School of Traditional Chinese Materia Medica 49#, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China;2. Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education, Wenhua Road 103, Shenyang 110016, China;3. Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;4. Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;5. School of Life Science and Technology, Dalian University, Dalian 116622, China;6. School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China |
| |
| Abstract: | The present paper describes the development of a simple and sensitive analytical method for quantification of Peptide T (PT) in rabbit plasma, using standard analytical equipment and on-line column enrichment, without prior extraction, clean-up or derivatization. The analytical procedure was found to be accurate, precise and linear. The accuracy was 100% (range 97–103%) and the mean precision was 8% (range 3–14%) for all (n=6) concentrations (0, 15, 50, 100 and 200 ng/ml). The total recovery was found to be approximately 80%, and it was found to be dependent upon the injection rate onto the extraction column. The correlation between added and found concentrations was 0.9982, and the limit of detection was estimated to be around 5 ng/ml. The method is therefore found to be suitable for bioavailability studies, involving Peptide T, in rabbits. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
