Effect of peptide to carrier ratio on the immune and ovarian response to inhibin immunization in cattle |
| |
| Institution: | 1. Animal Reproduction Department, Agriculture and Food Development Authority, Belclare, Tuam, Galway, Ireland;2. Physiology Division, Protein Metabolism Group, The Rowett Institute, Bucksburn, Aberdeen, AB2 9SB, UK;1. Department of Animal Genetics and Breeding, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141004, Punjab, India;2. Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141004, Punjab, India;1. Department of Animal Sciences, University of Stellenbosch, Matieland, 7602, South Africa;2. School of Agriculture and Environment, Faculty of Science, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia;3. Private Bag XI, Elsenburg, 7607, South Africa;1. ICAR-Central Institute of Fisheries Education, Panch Marg, Yari Road, Versova, Andheri West, Mumbai 400061, India;2. National Institute for Research in Reproductive Health (Indian Council of Medical Research), Jehangir Merwanji Street, Parel, Mumbai 400012, India;3. Bioinformatics Department, School of Biotechnology and Bioinformatics, D.Y. Patil University, CBD Belapur, Navi Mumbai 400614, India;1. School of Animal and Veterinary Sciences, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW, 2678, Australia;2. Graham Centre for Agricultural Innovation (NSW Department of Primary Industries and Charles Sturt University), Albert Pugsley Place, Wagga Wagga, NSW, 2650, Australia;3. Fred Morley Centre, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW, 2678, Australia;4. School of Agricultural and Wine Sciences, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW, 2678, Australia;5. Office of the Pro Vice-Chancellor (Research and Innovation) Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW, 2678, Australia |
| |
| Abstract: | We report on the effects of the peptide to carrier ratio on the immune and biological response to inhibin immunization in cattle. A peptide sequence from the αC-subunit of bovine inhibin was synthesized and conjugated to human serum albumin (HSA) at ratios of 4.3 moles (L) and 13.1 moles (M) of peptide per mole of HSA. Hereford-cross heifers (n = 6 per group) were injected with 3 mg of one of the peptide conjugates at primary, followed by a booster injection (1.5 mg) 11 weeks later. Control heifers (n = 6) were injected with HSA only. Blood samples were taken at regular intervals to measure antibody titre. Ovulation rate was measured by ultrasonography. Antibodies were generated in both peptide immunized groups. Control heifers and group L heifers had 1 ovulation at all ovulatory cycles monitored. Ovulation rate was increased (P < 0.05) in group M immunized heifers, with four of six heifers having twin ovulations in the first cycle following boost. These data support those of previous studies which indicated that immunization against the αC-subunit of bovine inhibin significantly disrupted the mechanism(s) controlling ovulation rate in cattle. It also indicates that both the immune and associated biological response is dependent on the nature of the conjugate used for immunization, specifically the ratio of peptide to carrier. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
