High-speed electrophoretic separation of DNA fragments using a short capillary |
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| Affiliation: | 1. Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai 200093, China;2. Photonics and Bio-medical Research Institute, Department of Physics Faculty of Science, East China University of Science and Technology, No. 130 Meilong Road, Shanghai 200237, China;3. Department of Applied Physics, Graduate School of Engineering, Osaka University, Yamadaoka Suita-city, Osaka 565-0871, Japan;1. Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu 221004, China;2. Department of Bioinformatics, Xuzhou Medical University, Xuzhou, Jiangsu 221004, China;3. Department of Chemistry, Zhejiang University, Hangzhou, Zhejiang 310058, China |
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| Abstract: | Capillary electrophoresis using a replaceable gel buffer was applied to the separation of DNA fragments. A short effective length capillary (1–2 cm) at low electric field allowed the separation of a 20–1000 bp ladder in 1 min. Although similar separation speed was achieved with a longer capillary at high field, the resolution of larger fragments was degraded. The short effective length capillaries were able to separate the wildtype and mutant PCR products of the TGF-β1 gene in under 45 s. |
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