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Construction of Kluyveromyces lactis killer strains defective in growth on lactic acid as a silage additive |
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| Authors: | Hiroko K Kitamoto Sadahiro Ohmomo Koichi Amaha Tomotaro Nishikawa Yuzuru Limura |
| Institution: | (1) National Institute of Agrobiological Resources, Kannondai, Tsukuba Ibaraki, Japan;(2) National Institute of Animal Industry, Kukisakimachi Ikenodai 2, Inashiki-gun Ibaraki, Japan;(3) National Grassland Research Institute, Senbonmatsu 768, Nishinasunomachi Nasugun Tochigi, Japan;(4) Department of Applied Chemistry and Biotechnology, Yamanashi University, Takeda, Koufu Yamanashi, Japan |
| Abstract: | Killer strain of Kluyveromyces lactis was constructed to prevent the aerobic deterioration of silage by disruption of KlPCK1 gene encoding phosphoenolpyruvate carboxykinase (PEPCK). The disruptants are defective in their ability of growth on lactic acid as a sole carbon source. The PEPCK activity was also deficient in the disruptants. The growth rate on lactose medium and the killing activity were equal to those of the parental strain. © Rapid Science Ltd. 1998 |
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