Specificity of antisera directed against rat liver cytochrome P-448 |
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Authors: | P E Thomas A Y Lu D Ryan S West W Levin |
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Affiliation: | Department of Biochemistry and Drug Metabolism Hoffmann-La Roche Inc. Nutley, New Jersey 07110, USA |
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Abstract: | A partially purified preparation of hepatic cytochrome P-448 from 3-methylcholanthrene treated rats was used to produce antisera in rabbits. Using both Ouchterlony double diffusion and quantitative immunoprecipitation analysis, this antisera was found to be more specific for cytochrome P-448 than for cytochrome P-450 from phenobarbital induced rats. The antisera did not form precipitin bands with the following rat liver microsomal proteins: cytochrome b5, NADH-cytochrome b5 reductase, NADPH-cytochrome c reductase or epoxide hydrase. |
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